Fig. 13

Immunohistochemical detection of Iba-1-positive microglia and NeuN-positive neurons in specific regions of juvenile-stressed and control groups. Representative double immunofluorescence images of the CA1 (a, a′), dAC L5/6 (b, b′), and M1 L5/6 (c, c′) are shown. NeuN-positive neurons are indicated by green fluorescence (Alexa Fluor 488), and Iba-1-positive microglia are indicated by red fluorescence (Alexa Fluor 594). Double confocal images of Iba-1 and NeuN reactivity in control (a–c) and stress mice (a′–c′) are shown. Scale bar = 50 μm in c′ (applies to a–c, a’–c’). Abbreviations are the same as those in Fig. 8