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Fig. 12 | BMC Neuroscience

Fig. 12

From: Juvenile stress induces behavioral change and affects perineuronal net formation in juvenile mice

Fig. 12

Immunohistochemical detection of Cat-315-positive PNNs and WFA-positive PNNs in specific regions of the juvenile-stressed and control groups. Representative double immunofluorescence images of the dAC L5/6 (a, a′, b, b′), and M1 L5/6 (c, c′, d, d′) are shown at high magnification. Double confocal images of Cat-315 and WFA reactivity in control (a, a′, c, c′) and stress mice (b, b′, d, d′) are shown. Cat-315-positive PNNs are indicated by green fluorescence (FITC), and WFA-positive PNNs are indicated by red fluorescence (Alexa Fluor 594). Scale bar = 10 μm in d′ (applies to ad, a′d′). The densities of Cat-315-positive PNN in the hippocampus (CA1, CA3, and DG) (e), and primary motor cortex (f) are shown. The percentages of Cat-315-positive PNNs co-localized with WFA-positive PNNs in the hippocampus (CA1, CA3, and DG) (g), and primary motor cortex (h) are shown. All data are presented as the mean ± SEM. *Significant difference from control mice (p < 0.05). The p values indicate two-way ANOVA by Bonferroni t test. Abbreviations are the same as those in Fig. 8. e Hippocampus; group: F1,54 = 1.000, region: F2,54 = 14.729, group × region: F2,54 = 1.155. CA1: p = 0.0832. CA3: p = 0.7439. DG: p = 0.7692, f M1; group: F1,36 = 0.770, region: F1,36 = 42.960, group × region: F1,36 = 0.172. M1 L2/3: p = 0.7455. M1 L5/6: p = 0.3670, g hippocampus; group: F1,54 = 5.000, region: F2,54 = 0.0339, group × region: F2,54 = 0.9348. CA1: p = 0.2881. CA3: p = 0.1203. DG: p = 0.2272, f M1; group: F1,36 = 2.943, region: F1,36 = 66.461, group × region: F1,36 = 0.5534. M1 L2/3: p = 0.4347. M1 L5/6: p = 0.1105

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