Calcium currents in striatal fast-spiking interneurons: dopaminergic modulation of CaV1 channels

BMC Neuroscience

Table 1 Contribution in percentage of the whole-cell Ca²⁺ current for each class of Ca²⁺ channel

Antagonist	Nicardipine	ω-conotoxin GVIA	ω-Agatoxin TK	SNX-482	TTA-P2
Concentration	20 µM	1 µM	1 µM	1 µM	1 µM
Ca²⁺ channel antagonist	Ca_V1	Ca_V2.2	Ca_V2.1	Ca_V2.3	Ca_V3
% of current blocked (mean ± S.E.M)	38 ± 1.1	23.4 ± 0.7	11.1 ± 1.4	20 ± 2	7.4 ± 2.3
n	12	14	14	6	13
p	0.0001	0.0001	0.0033	0.0009	0.0202

The first row (not counting the title) indicates the Ca²⁺ channel antagonist used. The second row contains saturating concentrations used. Third row stands for the specific Ca²⁺ channel class that was blocked. The fourth row displays the mean ± SEM of Ca²⁺ current blocked in percentage by each antagonist. Antagonists were tested alone or in sequence in different orders. Percentages were obtained from a system of linear equations that used data from all experiments (see Materials and methods). The fifth row shows samples size: the number of neurons tested with each antagonist. The sixth row indicates statistical significance or P-value of percentage blockade by each antagonist as compared to whole-cell current average before adding any antagonist (Kruskal–Wallis ANOVA with post hoc Dunn’s test of each paired comparison against the control: whole-cell Ca²⁺ current)

ISSN: 1471-2202