Fig. 1

Whole-cell Ca²⁺ currents in acutely dissociated FSI. a Schematic infection protocol in PV-cre mice with a viral construction containing tdTomato into the dorsal striatum. b Representative images of virally infected, acutely dissociated PV-cre FSI. Left: light microscopy; right: the same fluorescent tdTomato PV-cre cell. c Inward currents (bottom) elicited by rectangular voltage commands from − 80 to 50 mV (top) in 10 mV steps (tail currents are clipped). Empty circle shows where the amplitude current measurements were obtained. d Inward current in the same neuron elicited by a ramp command from − 80 to 50 mV (0.7 mV/ms). e Current–voltage relationship (I–V plot). Empty circles are measurements taken from currents elicited with voltage commands (as in c) and continuous line was the current obtained with the ramp command (as in d). Measurements using both protocols are superimposed. Note that measurements using the ramp command appear to “fit” measurements using the square commands suggesting good voltage control and space clamp. f  Representative time course of Ca²⁺ current blockade during bath application of 200 µM Cd²⁺