Fig. 6

Inhibited macroautophagy is not associated with increased ubiquitin levels in oligodendroglia treated with α-syn. Intracellular amounts of ubiquitin were measured in oligodendroglial cells exposed to extracellular sol or fib α-syn using Western blot analysis. Ubiquitin levels were normalized to actin levels (a). Furthermore, co-localization of ubiquitin and α-syn was analyzed using immunocytochemistry, labelling human α-syn (15G7) red and ubiquitin green (b). A slight increase of ubiquitin levels was measured upon treatment with sol and fib α-syn and BAF compared to untreated oligodendroglial cells. However, only fib α-syn treatment alone induced a significant increase of ubiquitin levels (a). Furthermore, no co-localization of ubiquitin staining (green) and α-syn (red) was found upon treatment with BAF and sol or fib α-syn. Scale bar 20 µm (b). Two-way analysis of variance with post hoc Bonferroni test was applied. Data are presented as mean ± SEM. *p < 0.05. N number equals 4