Fig. 1
Incorporation of α-syn by murine primary oligodendroglia as compared to the human oligodendroglioma cell line MO 3.13. Murine primary oligodendroglial cells (primary oligos) and the human oligodendroglial cell line MO 3.13 were treated with 18 µg/mL sol and fib α-syn for 24 h. Immunocytochemistry for α-syn (15G7, red) and PDGFRα (green) were performed and the evaluation was done by an unbiased investigator. The number of α-syn-positive oligodendroglial cells was evaluated and the percentage of α-syn-positive cells was calculated showing no difference between primary and MO 3.13 oligodendroglia. Arrows showing inclusions positively stained for 15G7 in the cytoplasm of primary and MO 3.13 oligodendroglial cells (a). The number of α-syn-positive inclusions per cell was counted revealing fewer inclusions per cell upon fib α-syn compared to sol α-syn exposure in both cell types (b). Moreover, the total area of inclusions per cell (µm2) was measured indicating a slight increase of the area in MO 3.13 compared to primary oligodendroglia; however, the difference did not reach statistical significance (c). Two-way analysis of variance with post hoc Bonferroni test was applied. Data are presented as mean ± SEM *p < 0.05; ***p < 0.001. Scale bar 20 µm. N number equals 4