Fig. 3

27-OHC does not kill cells and increases α-synuclein protein levels while mRNA levels remain unchanged. a Lactate Dehydrogenase assay shows that varying concentrations of 27-OHC do not elicit cell death relative to control untreated cells. Representative western blot (b) and optical density (c) of α-syn. Western blots are expressed as fold change over β-Actin. The amount of α-syn significantly increases with increased 27-OHC concentrations. d Real-time rt-PCR shows that 27-OHC does not increase SNCA mRNA. Data are expressed as individual values with mean ± SEM and includes determinations made in one experiment with (n = 3) and three technical replicates for LDH assay, two separate experiments with (n = 3) and three technical replicates for western blots, and one experiment with (n = 3) and three technical replicates for Real Time- rtPCR. **p < 0.01, ***p < 0.001 versus control