Fig. 1

Human dopaminergic neurons express Tyrosine Hydroxylase. Bright field microscopy of human dopaminergic neuronal precursor cells one day prior to the start of differentiation (A) and 25-day post differentiation (B). Immunofluorescence staining showing that the neurons express tyrosine hydroxylase, the rate limiting enzyme in dopamine synthesis (C; green) suggesting that these neurons are predominantly of dopaminergic origin. (D) is immunostaining with the neuron specific β-III Tubulin (red), and (E) is nuclear counterstain with DAPI (blue). F Overlay of tyrosine hydroxylase, neuron specific β-III Tubulin, and DAPI staining showing multiple neurons with nuclear and axonal/dendritic staining for tyrosine hydroxylase (arrows)