Skip to main content
Fig. 6 | BMC Neuroscience

Fig. 6

From: Chronic oxycodone induces axonal degeneration in rat brain

Fig. 6

Opioids activate pro-apoptotic signaling in MCF7 cells. a Western blot analysis of Bax and ATF4 in MCF7 cells treated with opioids and naloxone. Left panel, representative images of western blots of Bax, ATF4 and actin in MCF7 cells treated with buffer, 10 μM oxycodone (Oxy), 10 μM morphine (Morph), or 10 μM DAMGO in the presence (+) or absence (−) of 10 μM naloxone (Nx) for 24 h. Right panel, graphs of the densitometric analysis of Bax and ATF4. The graphs represent the mean ratio of signal of Bax and ATF4 to actin. Open bars—Bax; grey filled bars—ATF4. All data were normalized to buffer samples set as 1 (± SEM, n = 3). ATF4: oxycodone versus buffer, p < 0.02; morphine versus buffer, p < 0.03; and DAMGO versus morphine, p < 0.01. Bax: oxycodone versus buffer, p < 0.02; morphine versus buffer, p < 0.05; and DAMGO versus buffer, p < 0.05. b Western blot analysis of ATF4 and Bax in MCF7 cells treated with opioids and CTAP or naltrindole. Upper panel, representative images of western blots of ATF4, Bax and Actin in MCF7 cells treated with buffer, 10 µM oxycodone, 10 µM morphine, or 10 µM DAMGO in the presence or absence of 0.3 µM CTAP or 0.3 µM naltrindole (Nd) for 24 h. Lower panel, graphs of the densitometric analysis of ATF4 and Bax western blots. The graphs represent the mean ratio of signal of ATF4 and Bax to actin. Open bars—Bax; grey filled bars—ATF4. All data were normalized to buffer samples set as 1 (± SEM, n = 3). ATF4: oxycodone versus buffer, p < 0.03; morphine versus buffer, p < 0.04; DAMGO versus buffer, p < 0.02; and oxycodone + Nd versus oxycodone, p < 0.05. Bax: oxycodone versus buffer, p < 0.03; morphine versus buffer, p < 0.02; and DAMGO versus buffer, p < 0.05

Back to article page