Dissecting the pathobiology of altered MRI signal in amyotrophic lateral sclerosis: A post mortem whole brain sampling strategy for the integration of ultra-high-field MRI and quantitative neuropathology

Table 3 Primary antibodies

Antibody	Host	Staining dilution	Supplier	Cat. no.	Remarks	References
CD68 (PGM-1)	Mouse	1:50	DAKO	M0876	Marker for activated microglia and macrophages	[79, 81]
Ferritin	Rabbit	1:3000	Sigma	F5012	Iron storage protein	[79, 83, 84]
GFAP	Rabbit	1:2000	DAKO	Z0334	Astroglia marker	[79, 80]
Iba1	Rabbit	1:1000	WAKO	019-19741	Microglia marker	[81]
PLP	Mouse	1:1000	Bio-Rad	MCA839G	Major myelin protein	[82]
pTDP-43 (S409/410 -clone 9-11)	Mouse	1:40 000	Cosmo Bio	TIP-PTD-M01	Phosphorylated TDP-43	[27, 85, 86]
SMI-311	Mouse	1:1000	Biolegend	837801	Pan neuronal marker that recognise non-phosphorylated epitopes of neurofilament heavy and medium chains. Stains pyramidal neurons	[87,88,89]
SMI-312	Mouse	1:2000	Biolegend	837901	Pan axonal neurofilament marker that recognise phosphorylated epitopes of neurofilament heavy and medium chains	[87, 90]

ISSN: 1471-2202