Fig. 1

Experimental design. Hippocampal slices were prepared from postnatal day 6 and grown for 9 days in vitro. Tissue was collected (arrows) and snap frozen for RNA transcript analysis at 4 h of incubation with either lipopolysaccharide (LPS, 1 µg/ml)) or dobutamine or a combination thereof (paradigm 1). Alternatively, slices were incubated with either lipopolysaccharide (LPS, 1 µg/ml)) or dobutamine or a combination thereof for 24 h and thereafter incubated under conditions of oxygen–glucose deprivation (OGD) for 15 min with an additional 2 h of recovery in normoxic and normoglycemic conditions. Twentyfour hours after stimulation (normoxic control or OGD), protein levels of SOD3 were determined by ELISA (paradigm 2)