Fig. 6

Mechanical stress with lack of sleep bouts results in increased death. a Loss of daf-16 does not alter sleep quantity in animals lacking aptf-1. Animals were reared at 25 °C and assayed at 22 °C. Total time in sleep bouts reported in minutes. Box shows two middle quartiles, horizontal line indicates mean, bars represent maximum/minimum. Statistical significance by student’s two tailed t-test noted as * for p ≤ 0.001 versus wild type. Wild type n = 10, daf-16(mgDf50) n = 9, aptf-1(gk794) n = 10, daf-16(mgDf50);aptf-1(gk794) n = 11. b Mechanical stress, in combination with lack of sleep bouts, results in death. daf-16 (mgDf50), aptf-1(gk794), and daf-16(mgDf50);aptf-1(gk794) animals were selected at the beginning of the L4 lethargus. To deprive animals of sleep, animals were physically stimulated in the tail region using a platinum wire upon entering a L4/A lethargus sleep bout, as marked by cessation of locomotion and pharyngeal pumping. These animals are referred to as “poked”. Yoked animals were physically stimulated with the same force whenever poked animals were prodded, regardless if in sleep or motion bout. Stimulation continued over a roughly 40 min time interval and ended when poked animals no longer responded to stimulus. Control animals were not stimulated. Survival was determined 24 h after stimulation based on ability to move and/or feed. Animals: n = 23 for all genotypes. p ≤ 0.0001 (with 2 degrees of freedom) as assessed by chi square test