Fig. 1

Notch ligand lag-2 regulates sleep bout quantity and arousal threshold during L4/A lethargus. a Partial loss of lag-2 resulted in increased sleep. Box shows two middle quartiles, horizontal line indicates mean, bars represent maximum/minimum; * denotes statistical significance with p ≤ 0.01 versus wild type by student’s two tailed t-test. n = 13, except 14 for lag-2(q420) at 15 °C. b Partial loss of lag-2 increases sleep during the final stages of lethargus. Time in sleep bout shown during L4/A lethargus, split into 45 min intervals/quartiles: I, II, III, IV, and “After”. Significance *p ≤ 0.05 for wild type versus lag-2(q420); other time points not significant based on multiple t-tests comparison followed by a Bonferroni correction; error bars represent SEM. Four independent trials combined; 40 animals per genotype for each time point. c Loss of lag-2 function increased average pause duration in the last stages of lethargus. Significance and analysis indicated as in panel B. n = 13 for both genotypes. d Partial loss of lag-2 increases sleep bout frequency during final stages of L4/A lethargus. Significance and analysis indicated as in Panel B. e Decreased lag-2 function increased time in sleep bouts. Percent sleeping animals responding during 45 min intervals sequentially named I, II, III, and IV. Four independent trials combined here, totaling 40 animals per genotype at each time point. p ≤ 0.001 assessed as in panel B; error bars represent SEM. f Over-expression of lag-2 increased time in sleep bouts. hsp::lag-2 is leaky even at room temperature. Significance as in Panel A. hsp::empty n = 23, hsp::lag-2 n = 25. g Over-expression of LAG-2 resulted in increased arousal thresholds during sleep bouts. Animals carry hsp::empty or hsp::lag-2 transgenes. Significance as in Panel B. Results from three independent trials reported, 30 animals total each