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Fig. 1 | BMC Neuroscience

Fig. 1

From: Characterisation of Zika virus infection in primary human astrocytes

Fig. 1

a Viral titres in culture supernatant from HBCAs infected with ZIKV-Af and ZIKV-Br and collected at 0, 1, 2, 3, 4 and 9 days p.i. were determined by plaque assay using Vero cells. Viral titres are expressed as p.f.u./ml. Data are from two independent experiments done in triplicates and represent mean ± SEM. b HBCAs infected with different doses of ZIKV-Af and ZIKV-Br were grown and fixed on slides at day 2 p.i. were stained with anti-flavivirus envelope antibody (green) and counterstained with DAPI (blue). Bar, 50 µm. c Mock-infected HBCAs stained with primary and secondary antibodies were used as a negative control, and did not exhibit any ZIKV antigen staining. Bar, 50 µm. d The percentage of HBCAs that were positive for ZIKV antigen in culture on day 2 p.i. Data were obtained based on a total of 23,000 cells per group counted in at least ten independent fields. Data were obtained from two independent experiments done in triplicates with two different batches of astrocytes, are expressed as mean ± SEM and were analysed using Two-way ANOVA (GraphPad Prism 5.04). **p < 0.01. e Expression of GFAP, a marker of astrocytes, was demonstrated in HBCA culture stained with anti-GFAP antibody (green) and counterstained with DAPI (blue). Bar, 50 µm

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