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Fig. 3 | BMC Neuroscience

Fig. 3

From: Solid lipid curcumin particles provide greater anti-amyloid, anti-inflammatory and neuroprotective effects than curcumin in the 5xFAD mouse model of Alzheimer’s disease

Fig. 3

SLCP inhibits Aβ fibril formation greater than does Cur. Aβ42 peptide was dissolved in HFIP, and after being completely dried, it was dissolved in 60 mM NaOH and diluted with Tris buffer saline (1 mM, pH 7.4), yielding a 10 μM concentration. Fifty microliter of peptide solution was incubated with, or without, different concentrations of Cur or SLCP for 8- and 24-h. After the stipulated period for each experiment, about 10 μL of peptide solution was spotted on the nitrocellulose membrane. The blots were probed with Aβ-oligomer specific (A11) and fibril specific (OC) antibodies. ac inhibition of Aβ42-oligomer formation by Cur and SLCP. df Aβ42 fibril formation was attenuated by Cur and SLCP after 24 h of incubation. Note that SLCP showed greater inhibition of Aβ42 fibril formation than dietary Cur (d, e). Results were expressed as mean ± SEM from three independent experiments. **p < 0.01 and ***p < 0.001 in comparison to Aβ42-vehicle treated group

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