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Table 3 Candidate housekeeping gene comparisons

From: Evaluation of reference genes for gene expression studies in mouse and N2a cell ischemic stroke models using quantitative real-time PCR

Sample Mean ΔCp SD Mean SD Mean ΔCp SD Mean SD
   Tissues    Cells  
18S versus HPRT 9.190 0.555 0.568 9.515 1.056 0.877
18S versus β-actin 6.340 0.518   4.329 0.767  
18S versus Sdha 10.180 0.519   11.342 1.143  
18S versus GAPDH 19.700 0.665   19.096 0.723  
18S versus cyclophilin 6.060 0.584   4.657 0.699  
HPRT versus β-actin 2.853 0.613 0.572 5.185 0.543 0.840
HPRT versus Sdha 0.990 0.623   1.827 0.839  
HPRT versus GAPDH 10.510 0.681   9.581 0.960  
HPRT versus 18S 9.192 0.555   9.515 1.056  
HPRT versus cyclophilin 3.130 0.389   4.858 0.802  
Sdha versus HPRT 0.990 0.623 0.607 1.827 0.839 0.921
Sdha versus β-actin 3.843 0.642   7.013 0.688  
Sdha versus GAPDH 9.521 0.569   7.754 1.010  
Sdha versus 18S 10.182 0.519   11.342 1.143  
Sdha versus cyclophilin 4.120 0.681   6.685 0.926  
β-actin versus HPRT 2.853 0.613 0.623 5.185 0.543 0.671
β-actin versus Sdha 3.843 0.642   7.013 0.688  
β-actin versus GAPDH 13.363 0.634   14.767 0.736  
β-actin versus 18S 6.339 0.518   4.329 0.767  
β-actin versus cyclophilin 0.277 0.711   0.327 0.618  
Cyclophilin versus HPRT 3.130 0.389 0.626 4.858 0.802 0.734
Cyclophilin versus β-actin 0.277 0.711   0.327 0.618  
Cyclophilin versus Sdha 4.120 0.681   6.685 0.926  
Cyclophilin versus GAPDH 13.640 0.763   14.439 0.624  
Cyclophilin versus 18S 6.062 0.584   4.657 0.699  
GAPDH versus HPRT 10.510 0.681 0.662 9.581 0.960 0.811
GAPDH versus β-actin 13.363 0.634   14.767 0.736  
GAPDH versus Sdha 9.521 0.569   7.754 1.010  
GAPDH versus 18S 19.702 0.665   19.096 0.723  
GAPDH versus cyclophilin 13.640 0.763   14.439 0.624