Table 3 Candidate housekeeping gene comparisons
Sample | Mean ΔCp | SD | Mean SD | Mean ΔCp | SD | Mean SD |
|---|---|---|---|---|---|---|
Tissues | Cells | |||||
18S versus HPRT | 9.190 | 0.555 | 0.568 | 9.515 | 1.056 | 0.877 |
18S versus β-actin | 6.340 | 0.518 | 4.329 | 0.767 | ||
18S versus Sdha | 10.180 | 0.519 | 11.342 | 1.143 | ||
18S versus GAPDH | 19.700 | 0.665 | 19.096 | 0.723 | ||
18S versus cyclophilin | 6.060 | 0.584 | 4.657 | 0.699 | ||
HPRT versus β-actin | 2.853 | 0.613 | 0.572 | 5.185 | 0.543 | 0.840 |
HPRT versus Sdha | 0.990 | 0.623 | 1.827 | 0.839 | ||
HPRT versus GAPDH | 10.510 | 0.681 | 9.581 | 0.960 | ||
HPRT versus 18S | 9.192 | 0.555 | 9.515 | 1.056 | ||
HPRT versus cyclophilin | 3.130 | 0.389 | 4.858 | 0.802 | ||
Sdha versus HPRT | 0.990 | 0.623 | 0.607 | 1.827 | 0.839 | 0.921 |
Sdha versus β-actin | 3.843 | 0.642 | 7.013 | 0.688 | ||
Sdha versus GAPDH | 9.521 | 0.569 | 7.754 | 1.010 | ||
Sdha versus 18S | 10.182 | 0.519 | 11.342 | 1.143 | ||
Sdha versus cyclophilin | 4.120 | 0.681 | 6.685 | 0.926 | ||
β-actin versus HPRT | 2.853 | 0.613 | 0.623 | 5.185 | 0.543 | 0.671 |
β-actin versus Sdha | 3.843 | 0.642 | 7.013 | 0.688 | ||
β-actin versus GAPDH | 13.363 | 0.634 | 14.767 | 0.736 | ||
β-actin versus 18S | 6.339 | 0.518 | 4.329 | 0.767 | ||
β-actin versus cyclophilin | 0.277 | 0.711 | 0.327 | 0.618 | ||
Cyclophilin versus HPRT | 3.130 | 0.389 | 0.626 | 4.858 | 0.802 | 0.734 |
Cyclophilin versus β-actin | 0.277 | 0.711 | 0.327 | 0.618 | ||
Cyclophilin versus Sdha | 4.120 | 0.681 | 6.685 | 0.926 | ||
Cyclophilin versus GAPDH | 13.640 | 0.763 | 14.439 | 0.624 | ||
Cyclophilin versus 18S | 6.062 | 0.584 | 4.657 | 0.699 | ||
GAPDH versus HPRT | 10.510 | 0.681 | 0.662 | 9.581 | 0.960 | 0.811 |
GAPDH versus β-actin | 13.363 | 0.634 | 14.767 | 0.736 | ||
GAPDH versus Sdha | 9.521 | 0.569 | 7.754 | 1.010 | ||
GAPDH versus 18S | 19.702 | 0.665 | 19.096 | 0.723 | ||
GAPDH versus cyclophilin | 13.640 | 0.763 | 14.439 | 0.624 |