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Table 2 Reference gene expression stability values in the tissues and cells based on several programs

From: Evaluation of reference genes for gene expression studies in mouse and N2a cell ischemic stroke models using quantitative real-time PCR

Samples Rank geNorm NormFinder BestKeeper ΔCp General ranking
   Genes M Genes Stability index Genes SD Genes Average SD Genes
Tissues 1 HPRT 0.394 18S 0.193 HPRT 0.250 18S 0.568 HPRT
2 Cyclophilin 0.394 HPRT 0.206 Cyclophilin 0.340 HPRT 0.572 18 s
3 18S 0.512 Sdha 0.223 18S 0.370 Sdha 0.607 Cyclophilin
4 Sdha 0.558 β-actin 0.223 β-actin 0.460 β-actin 0.623 Sdha
5 β-actin 0.583 Cyclophilin 0.230 Sdha 0.520 Cyclophilin 0.626 actin
6 GAPDH 0.611 GAPDH 0.247 GAPDH 0.550 GAPDH 0.662 GAPDH
Cells 1 β-actin 0.618 β-actin 0.228 18S 0.400 β-actin 0.671 β-actin
2 Cyclophilin 0.618 Cyclophilin 0.310 Cyclophilin 0.410 Cyclophilin 0.734 Cyclophilin
3 GAPDH 0.670 GAPDH 0.367 GAPDH 0.480 GAPDH 0.811 GAPDH
4 18S 0.695 HPRT 0.419 β-actin 0.510 HPRT 0.84 18S
5 HPRT 0.753 18S 0.439 HPRT 0.640 18S 0.877 HPRT
6 Sdha 0.810 Sdha 0.451 Sdha 0.880 Sdha 0.921 Sdha