Fig. 2

Effect of compound 3 on LPS-induced iNOS and COX2 expression in BV-2 cells. BV-2 cells were pretreated with various concentrations (5, 10, and 20 µM) of compound 3 for 30 min and activated with 100 ng/mL LPS. After 6 h, the cells were collected for analysis of iNOS and COX-2 expression using western blot analysis (a–c). Similarly, LPS activation was made for 24 h as chronic BV2 activation and the iNOS and COX-2 expression was measured (d). α-Tubulin was used as the loading control. The densitometric analysis of bands was performed as described in the methods section. After 24 h, the culture medium was subsequently collected to measure the quantity of PGE2 (e), IL-6 (f), and TNF-α (g) using ELISA analysis. All data are presented as the mean ± standard error of the mean of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 indicates significant differences compared with treatment with LPS alone while ^###P < 0.001 indicates the significant differences compared with untreated control group. Here Ctl indicates untreated control and LPS indicates lipopolysaccharide