Table 1 Primer sequences forward and reverse primer sequences for real time qPCR and in situ hybridisation analysis of Eph/ephrinA mRNA expression in visual structures of P0 Ten-m3 KO mouse
From: Downstream mediators of Ten-m3 signalling in the developing visual pathway
Gene name | NCBI mRNA accession # | Primer sequences 5′–3′ |
|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | NM_001001303 | F_AACTTTGGCATTGTGGAAGG R_ACACATTGGGGGTAGGAACA |
EphrinA2 (Efna2) | NM_007909 | F_AGCATCAACGACTACCTGGA R_AAAAGGGGGTGAAGAGTTGG |
EphrinA5 (Efna5) | NM_010109.3 | F_AGCAACCCCAGATTCCAGAGG R_TTTTCCGAGAACTTCAGCG |
Eph receptorA5 (Epha5) | NM_007937.3 | F_TGCTATTCGGCACCTCTAAAGG R_GGGGGCATAGTTCTCATCAA |
Eph receptor A6 (Epha6) | NM_007938.2 | F_CTCCAGCCTTCCCTTCACC R_TATCTATCATCAAACTCCACACA |
Eph receptor A7 (Epha7) | NM_010141.3 | F_GCTGTAAATGGAGTTTCGGAC R_GTGTGGCAACATCAAGCCTA |
Zinc finger transcription factor 2 (Zic2) | NM_009574.3 | F_AATGGCTTATTGGCTTATTGG R_ACTTTGGCACGGCTCATATT |
Ephrin ligand B1 (EfnB1) | NM_010110.4 | F_ACCCGAGCAGTTGACTACCA R_ATGATGAGCAGGAAGATGACAC |
Eph Receptor B1 (EphB1) | NM_173447.3 | F_TGGCTATGGCAAGTTCAGTG R_CAATGTAGATCTTCATCCCT |