Fig. 2

Increased pCREB, pERK and neurogenesis in PDE4B1-D564A transgenic mice. All data in the bar graphs was obtained using ImageJ, where an increased signal is indicated by a lower intensity. All data are mean ± SE. Asterisks (*) show data from the transgenic mice that is statistically different from that obtained from wild-type mice. a The PDE4B1-D564A transgene increases hippocampal pCREB and neurogenesis. (i) Immunodetection of pCREB in wild-type (WT) and transgenic (TG) mice in hippocampus. (ii) Select areas within the hippocampus. Top pair of panels: Nissl staining of hippocampus shows no major change between wild-type (WT) and transgene (TG). Small boxes indicate regions of CA1 and dentate gyrus shown at higher magnification in the middle and lower panels. Middle 4 panels: CA1 region: Levels of pCREB are increased in the transgenic mice, while total CREB is not detectably different. Lower 4 panels: Dentate gyrus: Doublecortin (DC)–labeled cells and pCREB levels are increased in the transgenic mice. (iii) Quantitation of DC-labeled cells in dentate gyrus, as determined by ImageJ. b The PDE4B1-D564A transgene increases pCREB in piriform cortex. (i) Top pair: Representative images of piriform cortex. Arrows indicate the region of most intense immunoreactivity. Bottom pair: Regions used for quantitation of pCREB expression are identified by red lines. (ii) Quantitation of pCREB immunoreactivity in 4 brain regions (a, b), as determined by ImageJ