Fig. 2

Netrin-1-induced axon outgrowth in mouse E14 cortical neurons. Phase contrast images shown are representative of E14 neurons under basal conditions with few protrusions (a) and abundant in protrusions (b) on the axon shafts, and those after netrin-1 stimulation in the absence (c) or presence (d) of an anti-DCC function-blocking antibody. Netrin-1 was bath-applied to cultures at a concentration of 250 ng/mL for 4 h, with or without 1 μg/mL anti-DCC antibody applied 30 min before the addition of netrin-1. Scale bar, 20 μm