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Table 2 Intra and inter-run accuracy and precision of the method (n = 5)

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Concentration (ng/mL) Intra-run Inter-run
Accuracy R.S.D. (%) Accuracy R.S.D. (%)
Plasma
 Nicotine
  LLOQ 3 101.49 7.50 107.06 9.53
  LQC 9 93.36 5.02 98.92 9.33
  MQC 75 89.67 3.55 89.35 4.64
  HQC 150 92.48 5.90 98.61 11.30
 Cotinine
  LLOQ 3 84.34 3.7 94.29 10.99
  LQC 9 105.98 6.93 100.25 14.40
  MQC 225 90.55 1.24 89.96 6.97
  HQC 450 97.89 9.5 98.31 8.13
Brain
 Nicotine
  LLOQ 3 104.26 4.36 102.59 14.48
  LQC 9 111.01 4.93 107.28 5.65
  MQC 40 114.45 7.36 109.44 9.10
  HQC 80 113.46 6.91 110.70 7.51
 Cotinine
  LLOQ 3 96.66 8.59 98.55 8.01
  LQC 9 107.36 6.56 106.23 5.46
  MQC 40 106.64 9.68 109.61 8.20
  HQC 80 112.52 1.26 113.21 1.49