A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Table 1 Compound parameters for analytes and internal standard with MRM in positive electrospray mode

	Q1 mass (Da)	Q3 mass (Da)	DP	CE	CXP	EP
Analytes
Nicotine	163.2	132.1	100	27	10	10
Cotinine	177.2	98	100	34	10	10
Internal standard
Cotinine-D3	180.2	101.2	100	31	13	10

DP declustering potential, CE collision energy, CXP collision exit potential, EP entrance potential

ISSN: 1471-2202