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Table 1 Compound parameters for analytes and internal standard with MRM in positive electrospray mode

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

  Q1 mass (Da) Q3 mass (Da) DP CE CXP EP
Analytes
 Nicotine 163.2 132.1 100 27 10 10
 Cotinine 177.2 98 100 34 10 10
Internal standard
 Cotinine-D3 180.2 101.2 100 31 13 10
  1. DP declustering potential, CE collision energy, CXP collision exit potential, EP entrance potential