Fig. 2

Effects of PGC-1α on Aβ1–42 induced N2a cell death. a N2a cells were transfected with an emoty vector or PGC-1α overexpression plasmid followed by Western blot analysis of PGC-1α level. b N2a cells were transfected with an empty vector or PGC-1α overexpression plasmid and then treated with or without Aβ1–42 (25 μM) for 6 h. Cell lysates were harvested and immunoblotted for cleaved caspase-3. β-actin was used as loading control. c Representative pictures of TUNEL assay and Quantification of apoptotic cells by fluorescent TUNEL assay. d Cell viability was measured by CCK8. Results shown are demonstrated as mean ± SEM (two-way ANOVA with Bonferroni post-tests) and representative for three independent experiments. **p < 0.01, ***p < 0.001 compared to control group