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Table 2 mRNA levels of DGC components in differentiating oligodendrocytes (relative to GAPDH, n = 3)

From: Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy

Target gene Substrate Differentiation media Differentiation media Differentiation media  
Day 1 (10e−5) Day 3 (10e−5) Day 5 (10e−5) Day 1–5 pattern
Dystrobrevin-Alpha PDL 139 ± 10 340 ± 15 703 ± 19 Increase
  Lm-2 93 ± 6 305 ± 08 826 ± 54 Increase
Dystrobrevin-Beta PDL 54 ± 3 31 ± 2 32 ± 1 No change
  Lm-2 58 ± 7 22 ± 2 22 ± 2 No change
Dystroglycan PDL 3235 ± 217 3387 ± 168 5543 ± 245 No change
  Lm-2 3096 ± 406 3225 ± 455 3814 ± 225 No change
Dystrophin PDL 205 ± 0.5 360 ± 19 562 ± 34 No change
  Lm-2 216 ± 20 240 ± 4 410 ± 47 No change
Dystrophin related protein 2 (DRP2) PDL 106 ± 6 85 ± 5 110 ± 10 No change
  Lm-2 140 ± 7 51 ± 2 49 ± 4*** Decrease
Sarcoglycan-Alpha PDL 218 ± 27 328 ± 21 585 ± 13 Increase
  Lm-2 178 ± 2 243 ± 35 359 ± 18*** No change
Sarcoglycan-Beta PDL 1976 ± 70 2091 ± 75 3215 ± 49 Increase
  Lm-2 1776 ± 151 1614 ± 134 2048 ± 61*** No change
Sarcoglycan-Delta PDL 53 ± 3 26 ± 0.4 27 ± 0.5 Decrease
  Lm-2 41 ± 2 24 ± 0.6 32 ± 2 No change
Sarcoglycan-Epsilon PDL 175 ± 6 10 ± 2 139 ± 0.9 No change
  Lm-2 150 ± 6 99 ± 1 156 ± 6 No change
Sarcoglycan-Gamma PDL 27 ± 4 42 ± 4 73 ± 3 Increase
  Lm-2 25 ± 0.5 19 ± 1 41 ± 1*** No change
Sarcoglycan-Zeta PDL 57 ± 4 43 ± 4 38 ± 3 No change
  Lm-2 64 ± 6 28 ± 0.4 22 ± 2 No change
Syntrophin-Alpha1 PDL 44 ± 3 64 ± 3 100 ± 4 No change (p = 0.079)
  Lm-2 33 ± 5 48 ± 1 123 ± 9 Increase
Syntrophin-Beta1 PDL 251 ± 14 111 ± 6 132 ± 7 Decrease
  Lm-2 252 ± 9 121 ± 10 128 ± 9 Decrease
Syntrophin-Beta2 PDL 48 ± 6 41 ± 1 63 ± 5 No change
  Lm-2 16 ± 0.6 9 ± 0.3 10 ± 0.1 No change
Syntrophin-Gamma1 PDL 232 ± 21 106 ± 3 159 ± 5 No change
  Lm-2 115 ± 6 108 ± 4 141 ± 6 No change
Syntrophin-Gamma2 PDL 43 ± 2 18 ± 0.6 24 ± 2 No change
  Lm-2 24 ± 3 16 ± 0.6 28 ± 0.9 No change
Utrophin PDL 16 ± 0.4 12 ± 0.1 15 ± 0.8 No change
  Lm-2 16 ± 0.6 9 ± 0.3 10 ± 0.1 Decrease
  1. *** Significant differences in DGC component mRNA levels when comparing cells grown on PDL to those on Laminin
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