Fig. 4

Effects of combinations of ion⁺ channel inhibitors on oxidative stress and oligodendrocyte progenitor cells (OPCs). Mean ± SEM area above an arbitrarily set threshold a acrolein and b HNE immunointensity above an arbitrarily set threshold, in ventral optic nerve from normal, or injured vehicle or ion inhibitor treated animals, 3 days following partial optic nerve transection. Significant differences relative to vehicle are indicated by *p ≤ 0.05. c Representative images of acrolein and HNE immunointensity are shown; scale bar = 50 µm. d Mean ± SEM oligodendrocyte progenitor cell (OPC) counts in ventral optic nerve from normal, or injured vehicle or inhibitor treated animals, 3 days following partial transection. e Representative images illustrating OPC identification as NG2⁺/olig2⁺ cells; scale bar = 5 µm