Fig. 3

Co-expression of CLCA and ANO2 in olfactory cilia. a OSN under bright field, anti-CLCA immunofluorescence (green), anti-ANO2 immunofluorescence and overlay of three images; an inset of the apical region (rectangle) at higher magnification is shown on the right (N = 15); all fluorescent images were deconvolved. b Control in the absence of the primary antibodies. Neuron shown in bright field, immunofluorescence in the absence of anti-CLCA, immunofluorescence in absence anti-ANO2 and overlay of the three images (N = 12). Bar: 5 µm