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Fig. 5 | BMC Neuroscience

Fig. 5

From: Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures

Fig. 5

Dendrite formation in CREB-deficient cortical neurons. Cultured cortical neurons were transfected with either pTα1-EGFP (control, a) or the CRISPR/Cas9 vector together with pTα1-EGFP (CRISPR/Cas9, b). The cultures were treated with high KCl medium for 2 h at 7 DIV and fixed. The insets are enlarged images of cell bodies stained with anti-CREB antibody. Scale bar 50 μm. Quantitative analysis was carried out for CREB expression (c), dendrite length (d) and the number of dendrite tips (e). Bars represent the mean ± SEM (control, n = 25 cells; CRISPR/Cas9, n = 31 cells). Asterisks indicate a significant difference from control cells (Mann–Whitney’s U test; **P < 0.01)

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