Table 3 Immunohistology for activated and neuroprotected sensory neurons in healthy and diabetic rats after nerve reconstruction
Treatment | Immunohistology of dorsal root ganglia (DRG; L4/L5) | ||||
|---|---|---|---|---|---|
ATF-3 control (% of total sensory neurons) | ATF-3 experimental (% of total sensory neurons) | HSP-27 control (% of area in sections of DRG) | HSP-27 experimental (% of area in sections of DRG) | HSP-27 ratio Exp/Con | |
hCNG-Ihealthy (n = 5) | 1.4 [1.0–2.9] | 3.1 [2.9–4.0]c | 9.9 [7.6–10.1] | 16.5 [15.6–18.5]g | 1.9 [1.7–2.1] |
hCNG-Idiabetic (n = 11)k | 1.8 [1.1–2.6] | 4.6 [3.7–4.9]d | 6.6 [6.0–6.9] | 17.1 [15.3–19.8]h | 2.6 [2.4–3.0] |
CFeCNG-Ihealthy (n = 6)k | 1.5 [1.2–1.8] | 5.0 [4.9–6.1]e | 8.2 [6.5–10.6] | 16.4 [11.0–23.5]i | 1.7 [1.5–2.7] |
CFeCNG-Idiabetic (n = 7) | 2.3 [1.2–2.6] | 5.7 [5.0–6.0]f | 8.3 [5.4–9.1] | 18.2 [14.9–19.0]j | 2.2 [2.1–2.8] |
p-valuea (KW) | 0.77 | 0.001 | 0.06 | 0.84 | 0.034 |
Fisher’s methodb | |||||
hCNG-I/CFeCNG-I | NA | 0.0001 | NA | NA | 0.36 |
Healthy/diabetic | NA | 0.14 | NA | NA | 0.004 |