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Table 1 Overview of the experimental design of the two parallel sub-studies

From: Regeneration of long-distance peripheral nerve defects after delayed reconstruction in healthy and diabetic rats is supported by immunomodulatory chitosan nerve guides

Study

Observation time (days)

Experimental groups

n

Group name

Performed experiments

Study I (ULUND)

56

Healthy rats: Hollow chitosan nerve guide

5

hCNG-Ihealthy

Analysis of regenerative matrix and distal nerve segment: Presence of axons and activated and apoptotic Schwann cells by immunohistology; Analysis of sensory dorsal root ganglion activation and neuroprotection by immunohistology

Diabetic GK rats: Hollow chitosan nerve guide

11

hCNG-Idiabetic

Healthy rats: Chitosan film enhanced chitosan nerve guide

6

CFeCNG-Ihealthy

Diabetic GK rats: Chitosan film enhanced chitosan nerve guide

7

CFeCNG-Idiabetic

Study II (MHH)

150

Hollow chitosan nerve guide

10

hCNG-II

Motor recovery: Non-invasive electrophysiology (60, 90, 120, 150 days) and muscle weight ratio; Histomorphometry; Immunohistology

Chitosan film enhanced chitosan nerve guide

7

CFeCNG-II

  1. GK Goto-Kakizaki rats, hCNG hollow chitosan nerve guides, CFeCNG chitosan film enhanced chitosan nerve guides, I short-term experiments, II long-term experiments, ULUND University of Lund, MHH Medizinische Hochschule Hannover, i.e. Hannover Medical School