Study | Observation time (days) | Experimental groups | n | Group name | Performed experiments |
---|---|---|---|---|---|
Study I (ULUND) | 56 | Healthy rats: Hollow chitosan nerve guide | 5 | hCNG-Ihealthy | Analysis of regenerative matrix and distal nerve segment: Presence of axons and activated and apoptotic Schwann cells by immunohistology; Analysis of sensory dorsal root ganglion activation and neuroprotection by immunohistology |
Diabetic GK rats: Hollow chitosan nerve guide | 11 | hCNG-Idiabetic | |||
Healthy rats: Chitosan film enhanced chitosan nerve guide | 6 | CFeCNG-Ihealthy | |||
Diabetic GK rats: Chitosan film enhanced chitosan nerve guide | 7 | CFeCNG-Idiabetic | |||
Study II (MHH) | 150 | Hollow chitosan nerve guide | 10 | hCNG-II | Motor recovery: Non-invasive electrophysiology (60, 90, 120, 150 days) and muscle weight ratio; Histomorphometry; Immunohistology |
Chitosan film enhanced chitosan nerve guide | 7 | CFeCNG-II |