Skip to main content
Fig. 9 | BMC Neuroscience

Fig. 9

From: Regeneration of long-distance peripheral nerve defects after delayed reconstruction in healthy and diabetic rats is supported by immunomodulatory chitosan nerve guides

Fig. 9

Cell surface marker expression profiles of non-stimulated or M-CSF-stimulated macrophages differentiated on CFs or WGs. Macrophages, cultivated for up to 2 days under different conditions, were harvested and stained with CD68- (macrophage differentiation marker independent of M1/M2 phenotype), CCR7- (M1 phenotype), CD163- (M2a phenotype) and CD206-antibodies (M2a and M2c phenotype) followed by FACS analysis. a The gate was set on the FSC versus SSC to analyze monocyte-derived cells and exclude dead cells and cell debris. b Surface expression of CCR7 and intracellular expression of CD68. c Surface expression of CD163 and intracellular expression of CD68. d Surface expression of CD206 and intracellular expression of CD68 FSC forward scatter, SSC side scatter, WG well ground, CF chitosan film (n = 4 experiments)

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us