Fig. 3

Quantitative analysis of Pax6 and Cdca7 expression. A, B Representative images showing the mRNA expression of (A) Pax6 and (B) Cdca7. Sampling ribbons were drawn from medial cortex across the PSPB (broken lines) to ventral telencephalon and pixel intensities measured in the direction of the blue arrows. Background (BG) staining was measured in areas expressing neither gene. Scale bar 100 µm. C, D Average pixel intensity values along the ribbons in A and B, calculated by Fiji: the slopes of the regression lines dorsal and ventral to the PSPB were calculated. E, F Average values (±SEM) for slopes calculated as in C, D from multiple individuals (4 wild-type and Pax6 ^−/− embryos at E12.5 and at E14.5 and 3 embryos of each genotype at E13.5)