Fig. 5

Phospho-α-synuclein aggregate formation in toxin treated SH-SY5Y cells. SIRT1WT and SIRT1H363Y overexpressing SH-SY5Y cells were treated with toxin (20 or 10 μM diquat or 20 or 0.5 μM rotenone) and 0.2% PBS or DMSO; cells transfected with empty vector were used as a control. Cells were immunostained with phospho-α-synuclein. Images were captured through GFP filter under ×63 magnification. The captured images represent phospho-α-synuclein staining and the bar graphs represent the aggregate quantification in diquat or rotenone treated cells. Each bar represents % phospho-α-synuclein aggregates (±SD) from three independent assays (n = 3). ***p < 0.001, **p < 0.01 and *p < 0.05 when compared to 0.2% vehicle, one-way ANOVA (Bonferroni corrected), ^###p < 0.001 and ^##p < 0.01 when compared to control cells, ^~~~p < 0.001 and ^~p < 0.05 when compared to SIRT1WT overexpressing cells, two-way ANOVA (Bonferroni corrected). Scale bar 20 μM