Fig. 2

Expression of NF-κB in toxin treated SH-SY5Y. SIRT1WT and SIRT1H363Y were over-expressed in SH-SY5Y cells and control cells were transfected with empty vector following which cells were treated with diquat (a 20 or b 10 μM) or rotenone (c 20 or d 0.5 μM) for 20 h. Cells were harvested and the samples were probed for NF-κB. Data are presented as fold-untreated (+SD) from three independent assays (n = 3) with comparison to GAPDH as a housekeeping control protein. ***p < 0.001 when compared to 0.2% PBS, one-way ANOVA (Bonferroni corrected), ###p < 0.001 when compared to empty vector treatment, ^~~~p < 0.001, ^~~p < 0.01 and ^~p < 0.05 when compared to SIRT1WT cells, two-way ANOVA (Bonferroni corrected). Images are representative blot of NF-κB and GAPDH