Fig. 1

Effect of SIRT1 and its deacetylase activity on cell viability of toxin treated SH-SY5Y cells. SIRT1WT and SIRT1H363Y were over-expressed in SH-SY5Y cells and control cells were transfected with empty pLenti CMV vector following which cells were treated with diquat (20 or 10 μM) or rotenone (20 or 0.5 μM) for 20 h and viability was measured by reduction of Alamar Blue. Data are presented as mean % control ± SD from three independent assays (n = 3). ***p < 0.001 and **p < 0.01 when compared to 0.2% vehicle (PBS/DMSO), one-way ANOVA (Bonferroni corrected), ^###p < 0.001 and ^##p < 0.01 when compared to empty vector treatment and ^~~~p < 0.001 when compared to SIRT1WT, two-way ANOVA (Bonferroni corrected)