Fig. 1
KalSRKO mice exhibit enhanced locomotor sensitization to experimenter-administered cocaine. a Diagram showing the major Kalirin protein isoforms in adult rodents; the region of Kalirin shown to bind to the juxtamembrane region of GluN2B is shown. Sec14, phospholipid binding domain; DH, catalytically active Dbl homology GDP/GTP exchange factor domain; PH, pleckstrin homology domain; SH3, protein–protein interaction Src Homology 3 domain; Ig, immunoglobulin-like domain; FN, fibronectin-like domain; Kin, Ser/Thr kinase domain. b Diagram showing the genomic region surrounding Kalrn Exon 13 and the placement of forward (→) and reverse (←) primers used for genotyping WT, KalSRCKO and KalSRKO mice. c Adult male mice (N = 7 each, WT and KalSRKO) were studied every day for 2 weeks. Mice were initially handled for 3 days, injected with saline for 3 days (S1–S3), and then injected with cocaine i.p. [10 mg/kg, days 1 and 7; 20 mg/kg days 2–6 [9, 74]; C1–C7]; locomotor activity was recorded for 45 min after each injection. Mice were then maintained without injections for 12 days and tested again with 10 mg/kg cocaine (not shown). Males only, N = 7 each genotype. Statistics, SigmaPlot using repeated measures ANOVA, p < 0.001)