Fig.Ā 3From: Antagonistic action on NMDA/GluN2B mediated currents of two peptides that were conantokin-G structure-based designedEAR16 but not EAR18 shows selectivity for GluN2B over GluN2A. a, b EAR16-mediated inhibition of NMDA-evoked inward currents on HEK-293 cells expressing GluN1AāGluN2B subunits (a) or expressing GluN1AāGluN2A subunits (b). d, e EAR18-mediated inhibition of NMDA-evoked inward currents on HEK-293 cells expressing GluN1AāGluN2B (d) or in cells GluN1AāGluN2A subunits (e). c, f The areas of the NMDA-evoked inward currents were normalized to that measured prior to the addition of EAR16 (c) or EAR18 (f) (ā1: maximal inward current; 0: no current). No significant difference was found between ācontrol-NMDAā and āwash-NMDAā. Significant different compared to ācontrol-NMDAā (left symbol) or when compared to āwash-NMDAā (right symbol) #pĀ <Ā 0.0001, +pĀ <Ā 0.001, @pĀ <Ā 0.02, two-way ANOVA with Sidakās multiple comparison post-test; n (number of cells) EAR16, nĀ =Ā 4 for GluN1AāGluN2B, and nĀ =Ā 4 for GluN1AāGluN2A; EAR18 nĀ =Ā 3 for GluN1āGluN2B (washout nĀ =Ā 2) and nĀ =Ā 4 for GluN1AāGluN2ABack to article page