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Fig.Ā 2 | BMC Neuroscience

Fig.Ā 2

From: Antagonistic action on NMDA/GluN2B mediated currents of two peptides that were conantokin-G structure-based designed

Fig.Ā 2

EAR16 and EAR18 peptides inhibit NMDA-evoked inward currents in hippocampal neurons. a, b The inhibitory effect on NMDA-evoked currents during consecutive exposures to either 10, 100 and 500Ā ĀµM of EAR18 (a) and EAR16 (b). The areas of the NMDA-evoked inward currents were normalized to that measured prior to the addition of EAR18 (or EAR16) (āˆ’1: maximal inward current; 0: no current). Significant different compared to the control group. #pĀ <Ā 0.0001, +p<0.001, @pĀ <Ā 0.02, ^pĀ <Ā 0.01, $pĀ <Ā 0.05, two-way ANOVA with Dunnettā€™s multiple comparison post-test; n (number of cells) for each pulse were for Control (nĀ =Ā 7, 7, 7, 7, 6, 5, 4); for EAR18: 10Ā ĀµM (nĀ =Ā 7), 100Ā ĀµM (nĀ =Ā 8); 500Ā ĀµM (nĀ =Ā 5); for EAR16: 10Ā ĀµM (nĀ =Ā 10), 100Ā ĀµM (nĀ =Ā 6, 7, 7, 6, 6, 6, 6, 5) and 500Ā ĀµM (nĀ =Ā 7, 11, 11, 5, 5, 6, 5). c, d Show NMDA-evoked current traces before, in the presence of EAR16 (or ER18) and following washout. The arrows indicate that upon the simultaneous removal of NMDAĀ +Ā EAR, there is a transient increase in the NMDA-evoked inward current (see text for ā€œDiscussionā€ section). e Doseā€“response, the data was fitted to YĀ =Ā Bottom +Ā (TopĀ āˆ’Ā Bottom)/(1Ā +Ā 10^((LogIC50-x)*HillSlope)). When the entire current was considered: BottomĀ =Ā 0 no current, topĀ =Ā āˆ’1 (normalized maximal inward current), the IC50Ā s were 176 and 241Ā ĀµM for EAR18 and EAR16, respectively

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