Fig. 7
TNFalpha immunostaining (green) in dorsal horn deep lamina (DH) and ventral horns (VH) of the spinal cord at cervical 7 segmental level. NeuN, a nuclear stain, shown in red. A, E Representative immunoreactivity in FRC rat. B, F Representative immunoreactivity in untreated 12-week HRHF rats. Arrow in B indicates same cells as in upper inset, showing TNFalpha in NeuN stained neurons. Lower insets shows areas of assay for dorsal horn deep lamina and ventral horns, respectively. C, G Representative immunoreactivity in 12-week HRHF + IBU rat. Inset in C shows TNFalpha in NeuN stained neurons. D, H Representative immunoreactivity in 12-week HRHF-to-LRLF rat. Insets show TNFalpha staining in NeuN stained neurons. I, J Quantification of number of TNFalpha immunostained cells per mm2. **,*p < 0.05 and p < 0.01, compared to food restricted control (FRC) rats; ††p < 0.01, compared to age-matched untreated HRHF rats