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Fig. 5 | BMC Neuroscience

Fig. 5

From: Effectiveness of conservative interventions for sickness and pain behaviors induced by a high repetition high force upper extremity task

Fig. 5

Substance P and NK-1R immunostaining in superficial lamina of dorsal horns of spinal cords at cervical 7 segmental level. AD Representative images of dorsal horns showing punctuate substance P immunofluorescence (green) staining distributed across entire zone, medial to lateral (right to left side, respectively, in each panel). 12-week HRHF and HRHF + IBU rats show increased immunoexpression laterally. Insets in D shows location of substance P immunostaining at low power. Scale bars 50 μm, and applies to all panels except insets. EH Dorsal horns showing neurokinin-1 receptor (NK-1R) immunostaining (red). Inset in E shows localization of NK-1R immunostaining at low power. I, J Quantification of percent area with immunofluorescence staining for substance P and NK-1R, respectively, in C7 dorsal horn superficial lamina. Results for two-way ANOVAs are shown. *p < 0.05, compared to age-matched untreated FRC rats

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