Fig. 1

Primary cell cultures of neurons and astrocytes. The purity was estimated by immunostaining for specific protein markers. (a) Neuronal cultures were immunostained on glass slides against MAP2 protein (red), GFAP astrocyte specific protein (green) and DAPI (blue). The staining confirmed at least 90% purity of neuronal cultures from the mouse striatum. (b) Primary astrocytes stained against GFAP (green), MAP2 (red) and DAPI (blue). (c) Primary astrocytes stained against GFAP (green), NEUN (red) and DAPI (blue). (d) The enrichment of astrocytes was determined by flow cytometry of immunostained GLAST-positive cells isolated from the brains of 5 days old C57BL/6 J mice. Approximately 80% of the cells were GLAST-positive astrocytes after the separation on MACS columns