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Fig. 4 | BMC Neuroscience

Fig. 4

From: Hypertonic saline attenuates expression of Notch signaling and proinflammatory mediators in activated microglia in experimentally induced cerebral ischemia and hypoxic BV-2 microglia

Fig. 4

HS attenuates the expression of Notch-1 and NICD in hypoxia-activated BV-2 microglia. a The immunoreactive bands of Notch-1 (120 kDa), NICD (80 kDa) and β-actin (42 kDa), respectively. Bar graph b shows the protein expression of Notch-1 are significantly up-regulated in OGD BV-2 cells compared to control group (*P < 0.05); but it is moderately suppressed following treatment with 10 μM DAPT (#P < 0.05) and markedly suppressed following treatment with 80 mM HS (#P < 0.05). Bar graph c show the protein expression of NICD is significantly increased in OGD BV-2 cells compared to control group (*P < 0.05); however, it is decreased following treatment with 80 mM HS or 10 μM DAPT (#P < 0.05). The values represent the mean ± SD in triplicate. d The immunofluorescence of Notch-1 (G, J, M, P, red), lectin+ microglia (F, I, L, O, green) and the co-localization of Notch-1 in BV-2 microglia (H, K, N, Q). e The immunofluorescence of NICD (G, J, M, P, red), lectin+ microglia (F, I, L, O, green) and the co-localization of NICD in BV-2 microglia (H, K, N, Q). The results are consistent with the western blotting. Note that the expression of Notch-1 and NICD are increased in OGD-activated BV-2 cells and decreased with the treatment of 80 mM HS or 10 μm DAPT. HS hypertonic saline, DAPT N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine t-butyl ester, OGD oxygen glucose deprivation. NICD Notch intracellular domain. Scale bars in FQ 20 μm

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