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Fig. 2 | BMC Neuroscience

Fig. 2

From: Hypertonic saline attenuates expression of Notch signaling and proinflammatory mediators in activated microglia in experimentally induced cerebral ischemia and hypoxic BV-2 microglia

Fig. 2

10% HS attenuates the expression of RBP-JK and Hes-1 in peri-ischemic brain tissue. a The immunoreactive bands of RBP-JK (56 kDa), Hes-1 (37 kDa) and β-actin (42 kDa), respectively. Bar graphs b, c show the protein expression of RBP-JK and Hes-1 are significantly up-regulated in I/R and NS peri-ischemic brain tissues compared to the sham group (*P < 0.05) at 12 and 24 h; however, it is markedly attenuated following treatment with 10% HS at 12 and 24 h (#P < 0.05). The values represent the mean ± SD in triplicate. d The immunofluorescence of RBP-JK (G, J, M, P, red), lectin+ microglia (F, I, L, O, green) and the co-localization of RBP-JK and microglia (H, K, N, Q). e The immunofluorescence of Hes-1 (G, J, M, P, red), lectin+ microglia (F, I, L, O, green) and the co-localization of Hes-1 and microglia (H, K, N, Q). Note that the expression of RBP-JK and Hes-1 in activated microglia cells around the peri-ischemia cortex are markedly increased in I/R and NS rats and decreased following treatment with 10% HS 24 h. HS hypertonic saline, I/R ischemic–reperfusion injury, NS normal saline, RBP-JK recombination signal sequence-binding protein J, Hes-1 Hairy-Enhancer of Split-1. ns no significant difference. Scale bars in FQ 20 μm

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