Fig. 5
From: An immunohistochemical, enzymatic, and behavioral study of CD157/BST-1 as a neuroregulator
Enzymatic synthesis of cADPR and NAADP by FLAG-fusion proteins (mBST-1 and mCD38). a Western blot analysis of FLAG-fusion proteins from cell lysates of vector (50 µg), mouse BST-1 (mBST-1, 50 µg), and mouse CD38 (mCD38, 10 µg) using anti-FLAG antibody. b, c Enzyme activities for cADPR and NAADP synthesis were measured by incubation of purified FLAG-fusion proteins (vector, mBST-1 or mCD38) with appropriate substrates as described in the “Methods” section. *P < 0.001, **P < 0.0005, ***P < 0.0001. The mean ± S.E.M. of three independent experiments is shown