Fig. 3
From: Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury
Expression of c-Fos, PKCα and p-ERK/ERK protein levels in SC Spp1 knockdown and overexpression. a Western blot analysis of c-Fos, PKCα and p-ERK/ERK expression after Spp1 siRNA-858 transfection of SCs for 2 days, using GAPDH to normalize data to negative controls. The average of three independent experiments is shown as mean ± SEM. b–d Relative protein expression levels of c-Fos, PKCα, and p-ERK/ERK as determined by Western blot analysis. e Western blot analysis of c-Fos, PKCα, and p-ERK/ERK expression after pcDNA3.1-Spp1 plasmid transfection of SCs for 2 days, using GAPDH to normalize data to negative controls. The average of three independent experiments is shown as mean ± SEM. f–h Relative protein expression levels of c-Fos, PKCα, and p-ERK/ERK as determined by Western blot analysis