Fig. 2
From: Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury
Cytokine expression in SC Spp1 knockdown and overexpression. a Spp1 siRNA-1, 2, 3 transfection efficiency assay (*p < 0.05). b Real-time qPCR analysis of bax, bcl2, nf2, NT3, and PKCα mRNA expression levels after Spp1 siRNA-858 transfection of SCs for 2 days. GAPDH was used to normalize values to the negative control (*p < 0.05). The average of three independent experiments is shown as mean ± SEM. c Real-time qPCR analysis of bax, bcl2, nf2, NT3, and PKCα mRNA expression levels after pcDNA3.1-Spp1 plasmid transfection of SCs for 2 days, using GAPDH to normalize data to the negative control (*p < 0.05). The average of three independent experiments is shown as mean ± SEM