Fig. 2

Activity-dependent increase of BrdU⁺GSTπ⁺ cells in the sensorimotor cortex at day 10. a Confocal images of triple-labelled sections with antibodies against BrdU (red), NG2 (green) and GSTπ (blue). b Single confocal planes by z-stack co-labelling of BrdU⁺ with NG2⁺GSTπ⁺. c Graph depicting the total number of BrdU⁺NG2⁺GSTπ⁺ cells which increased after enriched environment housing and reaching training at 10 days compared to standard conditions. Bars represent mean ± SD. Asterisks indicate significant differences (P < 0.05). Scale bars represent a 100 µm and b 10 µm