Fig. 8
Overexpression of the ETA receptor increases ETB receptor expression in primary RGCs. a, b Representative images. Immunostaining showing the expression of ETA and ETB receptors in purified RGCs following transduction with either AAV-2-GFP or AAV-2-ETA. β-III-tubulin was used as a selective marker of RGCs. DAPI staining was used to visualize nuclei of RGCs. c, d Quantitative analysis of ETA receptor (c) and ETB receptor (d) expression in purified RGCs. Bars represent mean ± SEM (n = 3). Asterisks indicate statistical significance **p < 0.01 by student’s t-test