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Fig. 8 | BMC Neuroscience

Fig. 8

From: Selenium suppresses glutamate-induced cell death and prevents mitochondrial morphological dynamic alterations in hippocampal HT22 neuronal cells

Fig. 8

Co-localization of MitoTracker Green and LysoTracker Red. The cells were incubated for 30 min with MitoTracker Green (green color) and LysoTracker Red (red color) to visualize the colocalization of mitochondria and lysosomes. Colocalization of the two dyes turns the color into orange. Western blot detected LC3-I and II in the mitochondrial fraction. Relative LC3-II band intensities were measured and presented in the bar graph. LC3-II protein band intensity increased from 46.8 ± 1.7 in control to 105.4 ± 4.2 in glutamate and reduced to 57.4 ± 1.8 in selenite treated groups. F(2, 29) = 124.9. *p < 0.05 and **p < 0.01 versus control; ## p < 0.01 versus glutamate by ANOVA with post hoc Tukey’s test. N = 10 in each group

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BMC Neuroscience

ISSN: 1471-2202

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