Fig. 7

Western blotting of mitochondrial fission proteins. Fission proteins Fis-1, total Drp-1 and phospho-Drp1 (p-Drp1) were semi-quantified in the mitochondrial fractions using specific antibodies. COX IV served as an internal control. Band relative densities were measured and presented in the bar graphs. Fis-1 protein band intensity increased from 31.4 ± 1.3 in control to 51.1 ± 1.4 after glutamate incubation and reduced back to 40.5 ± 1.3 by selenite. F(2, 29) = 24.2. *p < 0.05 and **p < 0.01 versus control; ^# p < 0.05 and ^## p < 0.01 versus Glutamate by ANOVA host hoc Tukey’s test. Similarly, p-Drp-1 ban intensity increased from 12.3 ± 1.1 in control to 32.3 ± 1.4 in glutamate and declined to 16.6 ± 1.0 in selenite and glutamate co-treated cells. F(2, 29) = 54.7. *p < 0.05 and **p < 0.01 versus control; ^# p < 0.05 and ^## p < 0.01 versus Glutamate by ANOVA with post hoc Tukey’s test. N = 10 in each group